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Intracellular activity of sorangicins against S. aureus Newman in A549 ( A ) and NIH <t>3T3</t> ( B ) cells. At 1.75 hours post infection, cells were washed once and treated with different concentrations of rifampicin (RIF), sorangicin A (SorA), neosorangicin A (NeoSorA), or with 0.1% ( v/v ) methanol (control) in the presence of 10 µg mL −1 gentamicin. Reduction of bacterial burden was evaluated 24 hours post infection by CFU (colony-forming unit) counting. Limit of detection (LoD) is 10 1 CFU. Horizontal lines represent mean values of at least three independent biological experiments. Statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparison test. p < 0.05: *, p < 0.0001: ****.
Murine Fibroblast Nih 3t3 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Intracellular activity of sorangicins against S. aureus Newman in A549 ( A ) and NIH <t>3T3</t> ( B ) cells. At 1.75 hours post infection, cells were washed once and treated with different concentrations of rifampicin (RIF), sorangicin A (SorA), neosorangicin A (NeoSorA), or with 0.1% ( v/v ) methanol (control) in the presence of 10 µg mL −1 gentamicin. Reduction of bacterial burden was evaluated 24 hours post infection by CFU (colony-forming unit) counting. Limit of detection (LoD) is 10 1 CFU. Horizontal lines represent mean values of at least three independent biological experiments. Statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparison test. p < 0.05: *, p < 0.0001: ****.
Nih 3t3 Mouse Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nih 3t3 mouse fibroblast cells/product/ATCC
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murine embryonic fibroblasts cell line nih 3t3  (ATCC)
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ATCC murine embryonic fibroblasts cell line nih 3t3
Intracellular activity of sorangicins against S. aureus Newman in A549 ( A ) and NIH <t>3T3</t> ( B ) cells. At 1.75 hours post infection, cells were washed once and treated with different concentrations of rifampicin (RIF), sorangicin A (SorA), neosorangicin A (NeoSorA), or with 0.1% ( v/v ) methanol (control) in the presence of 10 µg mL −1 gentamicin. Reduction of bacterial burden was evaluated 24 hours post infection by CFU (colony-forming unit) counting. Limit of detection (LoD) is 10 1 CFU. Horizontal lines represent mean values of at least three independent biological experiments. Statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparison test. p < 0.05: *, p < 0.0001: ****.
Murine Embryonic Fibroblasts Cell Line Nih 3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/murine embryonic fibroblasts cell line nih 3t3/product/ATCC
Average 99 stars, based on 1 article reviews
murine embryonic fibroblasts cell line nih 3t3 - by Bioz Stars, 2026-03
99/100 stars
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mouse embryonic fibroblast cells  (ATCC)
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ATCC mouse embryonic fibroblast cells
Intracellular activity of sorangicins against S. aureus Newman in A549 ( A ) and NIH <t>3T3</t> ( B ) cells. At 1.75 hours post infection, cells were washed once and treated with different concentrations of rifampicin (RIF), sorangicin A (SorA), neosorangicin A (NeoSorA), or with 0.1% ( v/v ) methanol (control) in the presence of 10 µg mL −1 gentamicin. Reduction of bacterial burden was evaluated 24 hours post infection by CFU (colony-forming unit) counting. Limit of detection (LoD) is 10 1 CFU. Horizontal lines represent mean values of at least three independent biological experiments. Statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparison test. p < 0.05: *, p < 0.0001: ****.
Mouse Embryonic Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse embryonic fibroblast cells/product/ATCC
Average 99 stars, based on 1 article reviews
mouse embryonic fibroblast cells - by Bioz Stars, 2026-03
99/100 stars
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mouse fibroblast cell line nih 3t3  (ATCC)
99
ATCC mouse fibroblast cell line nih 3t3
Intracellular activity of sorangicins against S. aureus Newman in A549 ( A ) and NIH <t>3T3</t> ( B ) cells. At 1.75 hours post infection, cells were washed once and treated with different concentrations of rifampicin (RIF), sorangicin A (SorA), neosorangicin A (NeoSorA), or with 0.1% ( v/v ) methanol (control) in the presence of 10 µg mL −1 gentamicin. Reduction of bacterial burden was evaluated 24 hours post infection by CFU (colony-forming unit) counting. Limit of detection (LoD) is 10 1 CFU. Horizontal lines represent mean values of at least three independent biological experiments. Statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparison test. p < 0.05: *, p < 0.0001: ****.
Mouse Fibroblast Cell Line Nih 3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse fibroblast cell line nih 3t3/product/ATCC
Average 99 stars, based on 1 article reviews
mouse fibroblast cell line nih 3t3 - by Bioz Stars, 2026-03
99/100 stars
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Intracellular activity of sorangicins against S. aureus Newman in A549 ( A ) and NIH 3T3 ( B ) cells. At 1.75 hours post infection, cells were washed once and treated with different concentrations of rifampicin (RIF), sorangicin A (SorA), neosorangicin A (NeoSorA), or with 0.1% ( v/v ) methanol (control) in the presence of 10 µg mL −1 gentamicin. Reduction of bacterial burden was evaluated 24 hours post infection by CFU (colony-forming unit) counting. Limit of detection (LoD) is 10 1 CFU. Horizontal lines represent mean values of at least three independent biological experiments. Statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparison test. p < 0.05: *, p < 0.0001: ****.

Journal: bioRxiv

Article Title: Structure Elucidation, Biosynthesis and Biological Evaluation of Neosorangicin A, a Member of the Sorangicin Family

doi: 10.64898/2026.01.26.701680

Figure Lengend Snippet: Intracellular activity of sorangicins against S. aureus Newman in A549 ( A ) and NIH 3T3 ( B ) cells. At 1.75 hours post infection, cells were washed once and treated with different concentrations of rifampicin (RIF), sorangicin A (SorA), neosorangicin A (NeoSorA), or with 0.1% ( v/v ) methanol (control) in the presence of 10 µg mL −1 gentamicin. Reduction of bacterial burden was evaluated 24 hours post infection by CFU (colony-forming unit) counting. Limit of detection (LoD) is 10 1 CFU. Horizontal lines represent mean values of at least three independent biological experiments. Statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparison test. p < 0.05: *, p < 0.0001: ****.

Article Snippet: Human alveolar epithelial A549 cells (ATCC CCL-185) and murine fibroblast NIH 3T3 cells (ATCC CRL-1658), obtained from the American Type Culture Collection, were cultured in DMEM with 4.5 g/L glucose, 10% fetal bovine serum, 2 mM L -glutamine, 1mM sodium pyruvate and 1% non-essential amino acids at 37 °C and 7.5% CO 2 .

Techniques: Activity Assay, Infection, Control, Comparison